Transcription factors of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) family control important signaling pathways in the regulation of the host innate immune system. Various bacterial pathogens in the human gastrointestinal tract induce NF-kB activity and provoke proinflammatory signaling events in infected epithelial cells. NF-kB activation requires the phosphorylationdependent proteolysis of inhibitor of kB (IkB) molecules including the NF-kB precursors through ubiquitin-mediated proteolysis. The canonical NF-kB pathway merges on IkB kinases (IKKs), which are required for signal transduction. Using CRISPR-Cas9 technology, secreted embryonic alkaline phosphatase (SEAP) reporter assays and cytokine enzyme-linked immunosorbent assay (ELISA), we demonstrate that the actin-binding protein cortactin is involved in NF-kB activation and subsequent interleukin-8 (IL-8) production upon infection by Helicobacter pylori, Salmonella enterica and Pseudomonas aeruginosa. Our data indicate that cortactin is needed to efficiently activate the c-Sarcoma (Src) kinase, which can positively stimulate NF-kB during infection. In contrast, cortactin is not involved in activation of NF-kB and IL-8 expression upon infection with Campylobacter species C. jejuni, C. coli or C. consisus, suggesting that Campylobacter species pluralis (spp.) induce a different signaling pathway upstream of cortactin to trigger the innate immune response.  Kulcsszavak: ADP-heptose, NF-kB, Helicobacter pylori, T4SS, cortactin, Src, interleukin-8, inflammation