Egyszerű keresés   |   Összetett keresés   |   Böngészés   |   Kosár   |   Súgó  


Részletek

A cikk állandó MOB linkje:
http://mob.gyemszi.hu/detailsperm.jsp?PERMID=160558
MOB:2023/3
Szerzők:Hmissi, Souhir; Raddaoui, Anis; Frigui, Siwar; Abbassi, Mohamed Salah; Achour, Wafa; Chebbi, Yosra; Thabet, Lamia
Tárgyszavak:PSEUDOMONAS AERUGINOSA; ANTIBIOTIKUMOK; GYÓGYSZER-REZISZTENCIA; ÉGÉS
Folyóirat:Acta Microbiologica et Immunologica Hungarica - 2023. 70. évf. 3. sz.
[https://akjournals.com/view/journals/030/030-overview.xml]


  Detection of carbapenem resistant Pseudomonas aeruginosa co-harboring bla VIM-2 and bla GES-5 in burn patients / Souhir Hmissi [et al.]
  Bibliogr.: p. 203-205. - Abstr. eng. - DOI: https://doi.org/10.1556/030.2023.02089
  In: Acta Microbiologica et Immunologica Hungarica. - ISSN 1217-8950, eISSN 1588-2640 . - 2023. 70. évf. 3. sz., p. 199-205. : ill.


Pseudomonas aeruginosa is one of the major infectious agents in burn patients. Globally, high rates of antimicrobial resistance in P. aeruginosa have been reported, which is a cause of concern. The objective of this study was to determine the rate of resistance to carbapenems in P. aeruginosa isolates recovered from burn patients in Tunisia, to search genes encoding for carbapenemases and to determine their epidemiological markers (serotypes). A retrospective study was conducted in the Burn Intensive Care Unit (BICU) of the Trauma and Burn Centre of Ben Arous, Tunisia, and P. aeruginosa isolates collected from burn patients, from January to December 2018 were investigated. Carbapenemase screening was performed by Carbapenem Inactivation Method (CIM) and by EDTA-disk test for all carbapenem resistant isolates. Genes encoding carbapenemases (blaVIM, blaIMP, blaGES, blaNDM, and blaKPC) were investigated by PCR and selected carbapenemase genes were sequenced. During the study period, 104 non duplicated P. aeruginosa isolates were recovered. Most of them were isolated from skin samples (45.1%) and blood culture (22.1%) and belonged to O:11 (19.2%), O:12, and O:5 (12.5%, each) serotypes. High rates of resistance were observed for carbapenems (64.4%). Among the 67 carbapenem resistant isolates, 58 (86.5%) harbored blaVIM gene and 55 (82%) blaGES gene; in addition, 48 (71.6%) co-harbored blaVIM and blaGES genes. After sequencing, the blaVIM-2 and blaGES-5 gene variants were identified in seven randomly selected isolates. To the best of our knowledge, this is the first description of P. aeruginosa simultaneously harboring blaVIM-2 and blaGES-5 genes. Kulcsszavak: P. aeruginosa, burn patients, carbapenemases, VIM, GES