Aims: The purpose of our study was to conduct a phytochemical analysis of Rhaponticum carthamoides in order to gain a better understanding of its chemical profile and the active constituents responsible for its bioactivities. Methods: The methanolic extract of the root was fractionated using multiple chromatographic methods, including normal, medium and high-pressure chromatography, rotation planar chromatography with a variety of stationary phases and eluents. The structures of the isolated compounds were identified by comparing their NMR spectra with those reported in the literature. Results: As a result of our experiments, the mixture of cis and trans isomers of tiophene derivatives, vanillic acid, chloro-genic acid methyl ester, ajugasterone C, makisterone C, tachioside, 20-hydroxyecdysone, 24-epi-makisterone A and evofo-lin B were isolated and identified in the root of the plant. Conclusion: Tachioside, evofolin B and chlorogenic acid methyl ester have been reported for the first time from this species.  Kulcsszavak: Rhaponticum carthamoides, Leuzea carthamoides, tachioside, evofolin B, chlorogenic acid methyl ester